This is a knockout-validated antibody summary, based on the publication "A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blotting for CTSL and Integrin B4 (ITGB4, loading control, tested on the same gel) in wildtype, TMPRSS2- and CTSL-KO organoids. CTSL protein is completely lost in corresponding mutant organoids. From [1].

Mouse monoclonal IgG1

Company: ThermoFisher

Antibody: Cathepsin L

Catalog number: BMS1032

Summary: Mouse monoclonal antibody against human Cathepsin L. Reacts with human. Suitable for western blot, immunocytochemistry, immunohistochemistry (frozen), and ELISA.

Western blot

WT and CTSL-KO human intestinal organoids.

1:100 dilution overnight at 4C in PBS/10% milk protein/0.1% Tween20.

1:2,000 dilution anti-mouse HRP-conjugate (Dako, P0260) for 2h at 4C in the same buffer.

ECL (GE-Healthcare).

The same clone (33-2) is sold as Novus Biologicals NB100-1775; Santa Cruz Biotechnology sc-32320; Abnova MAB1432; Abcam ab6314, ab197278; Invitrogen MA1-26774, BMS1032.