This is a knockout-validated antibody summary, based on the publication "Formation of Lipofuscin-Like Autofluorescent Granules in the Retinal Pigment Epithelium Requires Lysosome Dysfunction", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. CTSD protein levels in ARPE-19 wild-type (WT) and mutant 19-CTSD-KO (CTSD-/-) cells assessed by Western blot. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: Cathepsin D

Catalog number: ab75852

Summary: Rabbit monoclonal antibody against a synthetic peptide within human Cathepsin D (aa 350 to the C-terminus). Reacts with mouse and human. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin and frozen) and flow cytometry (intra).

Western blot

WT and CTSD-KO ARPE-19 cells.

5% nonfat dry milk in Tris-buffered saline (TBS) (50 mM Tris, 150 mM NaCl, pH = 7.6) containing 0.1% Tween-20 (Sigma-Aldrich) (TBS-T) for 1 hour.

1:5,000 horseradish peroxidase-conjugated secondary antibody in blocking buffer for 2 hours at room temperature.

chemiluminescence ECL Prime Western Blotting Substrate (GE Healthcare, Chicago, IL) and imaged using a Chemidoc XRS (Bio-Rad).