β-Catenin antibody | knockout validation | Cell Signaling 9582

This is a knockout-validated antibody summary, based on the publication "The RNA genome of hepatitis E virus robustly triggers antiviral interferon response", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: β-Catenin

Catalog number: 9582

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to the carboxy terminus of human β-catenin. Reacts with human, mouse, rat, and monkey. It is predicted to reacts based on 100% sequence homology with Xenopus, bovine, dog, pig, and horse. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin and frozen).

Validation Method

Western blot

Sample

WT and β-catenin-/- MEF cells.

Secondary incubation

Anti-rabbit IRDye-conjugated antibodies were used as secondary antibodies for western blotting (Stressgen, Victoria, BC, Canada).

Figure

The β-catenin protein level was detected in WT and β-catenin-/- MEF cells. Please see Figure S8h in the article [1].

References
  1. Wang W, Wang Y, Qu C, Wang S, Zhou J, Cao W, et al. The RNA genome of hepatitis E virus robustly triggers antiviral interferon response. Hepatology. 2017;: pubmed publisher