This is a knockout-validated antibody summary, based on the publication "One-step generation of conditional and reversible gene knockouts", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1
Company: Cell Signaling
Antibody: β-Catenin
Catalog number: 4627
Summary: Mouse monoclonal IgG1 against a synthetic peptide corresponding to the carboxy terminus of human β-catenin protein. Reacts with human, and it is predicted to react based on 100% sequence homology with mouse, rat, monkey, and pig. Suitable for immunofluorescence/immunocytochemistry and flow cytometry.
Immunocytochemistry
Control and β-catenin-KO mouse embryonic stem cells (mESCs).
5% donkey serum (D9663, Sigma) and 0.1% Triton X-100 for 1 h at RT.
1:1,000 dilution in blocking buffer overnight at 4 °C.
Immunofluorescence of β-catenin before and after Cre transfection in mESCs. Please see Figure 2a in the article [1].
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- product
- Cell Signaling Technology β-Catenin (L54E2) Mouse mAb (IF Preferred)
- Cell Signaling Technology β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 488 Conjugate)
- Cell Signaling Technology β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 647 Conjugate)
- Cell Signaling Technology β-Catenin (L54E2) Mouse mAb (Biotinylated)
- Cell Signaling Technology β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 555 Conjugate)
- Cell Signaling Technology β-Catenin (L54E2) Mouse mAb (PE Conjugate)