α-Catenin antibody | knockout validation | BD Biosciences 610194

This is a knockout-validated antibody summary, based on the publication "Tumor-suppressor function of Beclin 1 in breast cancer cells requires E-cadherin", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

α-Catenin antibody | knockout validation | BD Biosciences 610194 figure 1
Figure 1. Western blot analysis of alpha-catenin in indicated cells after transduction with a CRISPR/Cas9 lentivirus containing either no (empty) or two sgRNAs targeting CTTNA1. From [1].
Antibody information

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: α-Catenin

Catalog number: 610194

Summary: Mouse monoclonal antibody against mouse α-Catenin (aa. 729-906). Reacts with human, mouse, rat, dog, and chicken. Suitable for western blot, immunofluorescence, and immunohistochemistry. Not recommended for immunoprecipitation.

Validation Method

Western blot

Sample

Control and alpha-catenin KO MCF7 cells.

Primary incubation

1:500 dilution.

References
  1. Wijshake T, Zou Z, Chen B, Zhong L, Xiao G, Xie Y, et al. Tumor-suppressor function of Beclin 1 in breast cancer cells requires E-cadherin. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher