This is a knockout-validated antibody summary, based on the publication "Combination of IAP antagonist and IFNγ activates novel caspase-10- and RIPK1-dependent cell death pathways", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1
Company: MBL
Antibody: Caspase-8
Catalog number: M058-3
Summary: Mouse monoclonal IgG1 against recombinant human Caspase-8 (180-480 aa, terminal C). Reacts with human. Suitable for western blot and immunoprecipitation.
Western blot
WT and CRISPR/Cas9-mediated CASP8−/− HT29 cells. Cells were lysed in DISC lysis buffer on ice for 20 min before the addition of SDS sample loading buffer or lysed directly in SDS lysis buffer (126 mM Tris-HCl, pH 8, 20% (v/v) glycerol, 4% (w/v) SDS, 0.02% (w/v) bromophenol blue, 5% (v/v) 2-mercaptoethanol) boiled and sonicated.
5% milk in PBST.
Diluted in 2% BSA in PBST.
Immunoblot of Caspase-8 in the indicated cells types after treatment with IFNγ/SM, QVD and Nec-1 or not treated for 24 h. Arrows indicate full-length and processing products of caspases. Please see Figure 5b in the article [1].
The same clone (5D3) is sold as LifeSpan Biosciences LS-C179790; MBL International M058-3.
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