This is a knockout-validated antibody summary, based on the publication "Executioner Caspase-3 and 7 Deficiency Reduces Myocyte Number in the Developing Mouse Heart", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of Caspase-3 and GAPDH (loading control) in hearts of wild type and caspase-3KO/caspase-7KO mice. From [1].

Mouse monoclonal IgG1

Company: Enzo Life Science

Antibody: Caspase-7

Catalog number: ADI-AMM-127

Summary: Mouse monoclonal IgG1 against recombinant human Caspase-7 protein (full length). Reacts with human, mouse, rat, hamster, and monkey. Suitable for western blot and immunohistochemistry (paraffin).

Western blot

P2 neonatal hearts from WT and Nkx2.5-driven caspase-3KO/caspase-7KO mice. Total protein extracts were obtained with Tris-buffered 2% SDS solution at pH 6.8.

1/1,000 dilution.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.