This is a knockout-validated antibody summary, based on the publication "Caspase-7: a critical mediator of optic nerve injury-induced retinal ganglion cell death", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Representative western blot of pro-caspase-7 in protein extracts from retinas from WT and Casp7 −/− mice. Beta-actin used as loading control. From figure [1].

Antibody: Caspase-7

Catalog number: 9492

Summary: Rabbit polyclonal raised against a synthetic peptide corresponding to residues surrounding the cleavage site of human caspase-7. Reacts with human, mouse, rat, and monkey in western blotting.

Company: Cell Signaling

Western blot

Protein extracts from retinas from WT and Casp7 −/− mice, using mammalian protein extraction buffer (Thermo Scientific, Rockford, IL) containing protease inhibitor and phosphatase inhibitor cocktails (Thermo Scientific).

5 % milk in Tris Buffered Saline Tween solution (TBST; 20 mM Tris, 0.5 M NaCl, and 0.05 % Tween 20, pH 7.4) for 1 h

1:1000 diluted in Super Block T20 (Thermo Scientific). at 4 C overnight

goat-anti-rabbit-horseradish peroxidase (1:10,000; Thermo Scientific, Cat # 32460) diluted in TBST for 1 h

visualized in a FluorChem 8900 imager (Alpha Innotech, San Leandro, CA) using ECL detection reagent Super Signal West Femto Maximum Sensitivity Substrate (Thermo Scientific).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.