This is a knockout-validated antibody summary, based on the publication "Executioner caspases and CAD are essential for mutagenesis induced by TRAIL or vincristine", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2a
Company: BD Biosciences
Antibody: Caspase-3
Catalog number: 610323
Summary: Mouse monoclonal IgG2a against human CPP32 (aa. 1-219). Reacts with human protein by western blot, immunofluorescence, and immunoprecipitation. Not recommended for immunohistochemistry.
Western blot
Control, caspase-3 single knockout and caspase-3/7 double knockout cells.
1% blocking reagent (Roche, Sydney, NSW, Australia) in PBS.
1:1,000 dilution.
1:20,000 dilution horseradish peroxidase (HRP)-conjugated secondary antibodies in block buffer containing 1% Triton X-100.
SuperSignalWest Dura Extended Duration Substrate (Thermo Fisher Scientific, Vic, Australia).
Caspase-3, -7 and -8 protein expression in control, single knockout and double knockout cells was assessed by immunoblot. Probing for GAPDH was used to indicate loading. From [1].
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