This is a knockout-validated antibody summary, based on the publication "Caspase-12, but Not Caspase-11, Inhibits Obesity and Insulin Resistance", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rat monoclonal IgG2a
Company: Sigma
Antibody: Caspase-12
Catalog number: C7611
Summary: Rat monoclonal IgG2a against recombinant mouse caspase 12 (residues 95-318). Reacts with mouse and rat by western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry, and microarray.
Western blot
Adipocytes from WT and Casp12-/- mice. Tissues were lysed in buffer B150 (20 mM Tris-HCl pH 8.0, 150 mM KCl, 10% glycerol, 5 mM MgCl2, and 0.1% NP40) supplemented with Complete-mini protease inhibitors (Roche Applied Science, Cat# 11836153001) and phosphatase inhibitors (Sigma Cat# S7920, 71768, G6376).
Western blot of Caspase-12 primary differentiated adipocytes from WT and
Casp12-/- mice treated with LPS. Please see Supplementary Figure 1a in the article [1].
The same clone (14F7) is sold as LifeSpan Biosciences LS-C179730; Abcam ab10455; Invitrogen MA1-24704; Sigma-Aldrich C7611.
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