This is a knockout-validated antibody summary, based on the publication "Caspase-11-mediated endothelial pyroptosis underlies endotoxemia-induced lung injury", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. Immunoblot analysis of Caspase-11 in endothelial cells isolated from lungs of Casp11fl/fl and Casp11EC–/– mice (n = 3) after the challenge with LPS (40 mg/kg i.p.) for 6 hours From [1].

Rat monoclonal IgG2a

Company: Novus Biologicals

Antibody: Caspase-11

Catalog number: NB120-10454

Summary: Rat monoclonal IgG2a against a recombinant p30 subunit of mouse Caspase 11. Reacts with human and mouse. Suitable for western blot, ELISA, immunocytochemistry/immunofluorescence, immunohistochemistry (frozen and paraffin), immunoprecipitation and microarray.

Western blot

Endothelial cells isolated from lungs of Casp11fl/fl and Casp11EC–/– mice. Cells were lysed with RIPA buffer.

5% nonfat dry milk in PBS with 0.1% Tween 20 (PBST, Sigma-Aldrich) for 1 hour at room temperature.

Overnight at 4 °C.

The same clone (17D9) is sold as Novus Biologicals NB120-10454, NB120-10454AF405, NB120-10454AF488, NB120-10454AF647, NB120-10454AF700, NB120-10454B, NB120-10454C, NB120-10454FR, NB120-10454G, NB120-10454H, NB120-10454IR, NB120-10454PCP, NB120-10454R, NB120-10454UV, NB120-10454V; Santa Cruz Biotechnology sc-56038; Invitrogen MA1-24703, 14-9935-80, 14-9935-82; Sigma-Aldrich C1354; GeneTex GTX10454.