This is a knockout-validated antibody summary, based on the publication "An endogenous caspase-11 ligand elicits interleukin-1 release from living dendritic cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rat monoclonal IgG2a
Company: Biolegend
Antibody: Caspase-11
Catalog number: 647202
Summary: Rat monoclonal IgG2a against a fusion protein of p30 of caspase 11. Reacts with mouse by western blot.
Western blot
WT and Caspase-11 KO dendritic cells (DCs). Cells were lysed in buffer containing 1% NP40, 50mM Tris-HCl (pH 7.4), 150mM NaCl. Protease inhibitors and phosphatase inhibitors were added just prior to cell lysis.
Western blot of Caspase-11 in WT and Caspase-11 KO cells. Actin was used as loading control. Please see Supplementary Figure 7h in the article [1].
The same clone (Cas11.17D9) is sold as BioLegend 647201, 647202; OriGene TA328073.
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