This is a knockout-validated antibody summary, based on the publication "Combination of IAP antagonist and IFNγ activates novel caspase-10- and RIPK1-dependent cell death pathways", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1ĸ

Company: MBL

Antibody: Caspase-10

Catalog number: M059-3

Summary: Mouse monoclonal IgG1ĸ against recombinant human full-length FLICE2. Reacts with human by western blot.

Western blot

WT and CRISPR/Cas9-mediated CASP8−/− HT29 cells. Cells were lysed in DISC lysis buffer on ice for 20 min before the addition of SDS sample loading buffer or lysed directly in SDS lysis buffer (126 mM Tris-HCl, pH 8, 20% (v/v) glycerol, 4% (w/v) SDS, 0.02% (w/v) bromophenol blue, 5% (v/v) 2-mercaptoethanol) boiled and sonicated.

5% milk in PBST.

Diluted in 2% BSA in PBST.

Immunoblot of Caspase-10 in the indicated cells types after 24 h treatments. Arrows indicate full-length and processing products of caspases. Please see Figure 6c in the article [1].

The same clone (4C1) is sold as LifeSpan Biosciences LS-C179791; MBL International M059-3.