This is a knockout-validated antibody summary, based on the publication "Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1β Protein during Virion-Mediated Activation of the AIM2 Inflammasome", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot assay for caspase-1 and GAPDH in THP-1 cells transduced with Cas9 and guide RNA (gRNA) targeting HUMCYC pseudogene or in indicated CRISPR-generated knockout cells. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Caspase-1

Catalog number: 3866

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues within the p20 subunit of human caspase-1. Reacts with human and it is predicted to react based on 100% sequence homology with monkey. Suitable for western blot and immunoprecipitation.

Western blot

Control and caspase-1-KO THP-1 cells. Cell pellets were lysed in RIPA buffer (10 mM Tris-Cl (pH 8.0), 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, 140 mM NaCl, and protease and phosphatase inhibitor cocktail).

5% milk in 1× phosphate-buffered saline (PBS) containing 0.1% Tween 20 for 30 min at room temperature.

Overnight at 4°C.

Horseradish peroxidase (HRP)-conjugated secondary antibodies in 5% milk for 30 min at room temperature.

Enhanced chemiluminescence (Thermo Fisher).