This is a knockout-validated antibody summary, based on the publication "Caspase-8 as an Effector and Regulator of NLRP3 Inflammasome Signaling", as cited below [2]. Another article "" also indicates the knockout validation for this antibody (figure 1) [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of caspase-11, caspase-1 and IL-1β proteins in total lysates (CL) and supernatants (SN) of the three groups of bone-associated mesenchymal stromal cells. from figure 2a of the article 29807832.

Mouse monoclonal IgG1

Company: Adipogen

Antibody: Caspase-1

Catalog number: AG-20B-0042

Summary: Mouse monoclonal IgG1 against recombinant mouse caspase-1. Reacts with endogenous full-length and activated (p20 fragment) mouse caspase-1 by western blot, immunohistochemistry (paraffin) and immunoprecipitation.

Western blot

Bone marrow-derived dendritic cells (BMDC) from WT and Casp1/11-/- mice. Whole cell detergent lysates were prepared by addition of 56 ul of RIPA lysis buffer (0.5% sodium deoxycholate, 0.1% SDS, 1% IgePal CA630 in PBS, pH 7.4, plus protease inhibitor mixture). Extracellular medium samples were concentrated by trichloroacetic acid precipitation/acetone washing.

1 ug/ml dilution.

0.13 ug/ml dilution HRP-conjugated secondary antibodies.

Chemiluminescent images of the developed blots were detected, stored, and quantified using a FluorChemE processor (Cell Biosciences).

Western blot analysis of caspase-1 in the extracellular media and cell lysates from WT and Casp1/11-/- BMDC primed with LPS and stimulated with nigericin for the indicated times. Please see Figure 1f in the article [2].