This is a knockout-validated antibody summary, based on the publication "Re-evaluation of protein kinase CK2 pleiotropy: new insights provided by a phosphoproteomics analysis of CK2 knockout cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2a
Company: Bio-Rad
Antibody: Casein Kinase 2 alpha
Catalog number: MCA3031Z
Summary: Mouse monoclonal IgG2a against a recombinant protein corresponding to aa 1 - 100 of human CSNK2A1. Reacts with human by western blot and immunofluorescence.
Western blot
Cells were lysed in ice-cold buffer containing 20 mM Tris–HCl (pH 8.0), 150 mM NaCl, 2 mM EDTA, 2 mM EGTA, 1% Triton X-100 (v/v), protease inhibitor cocktail Complete (Roche) and phosphatase inhibitor Cocktail 2 and 3 (Sigma).
Horseradish peroxidase-conjugated (PerkinElmer (Waltham, MA, USA)).
Chemiluminescence on a Kodak Image Station 440MM PRO.
Western blot analysis of CK2-substrates in wild type (WT)and CK2-KO cells (two different CK2α/α′(−/−) clones (A and B), and two different CK2β(−/−) clones (A and B). Please see Figure 5a in the article [1].
The same clone (3D9) is sold as Bio-Rad MCA3031Z; LifeSpan Biosciences LS-C196985; Abnova H00001457-M01; Sigma-Aldrich WH0001457M1.
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