This is a knockout-validated antibody summary, based on the publication "Calponin-3 is critical for coordinated contractility of actin stress fibers", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of Cnn3 in the indicated cells types. Anti-tubulin antibody was used as a control for sample loading. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: Calponin 3

Catalog number: sc-271188

Summary: Mouse monoclonal IgG1 (kappa light chain)against amino acids 275-329 mapping at the C-terminus of Calponin 3 of human and rat origin. Recommended for detection of Calponin 3 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot

Wild type, CNN3 silenced and CNN3 knockout cells U2OS cells. Cells were washed with ice-cold PBS, then lysed with 4xLSB-DTT.

5% BSA for 1 hour.

1:1,000 dilution.

Anti-mouse HRP secondary antibody (Invitrogen).

Western Bright ECL-spray (Advansta).