This is a knockout-validated antibody summary, based on the publication "Regulation of TET protein stability by calpains", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of WT, CAPN1-/- and CAPN2-/- mESC in day 8 embryonic body. Tubulin was used as loading control. From [1].

Rabbit polyclonal

Company: Cell Signaling

Antibody: Calpain2

Catalog number: 2539

Summary: Rabbit polyclonal against a synthetic peptide corresponding to the human sequence of calpain 2 (large subunit). React with human, mouse, and rat. Suitable for western blot, immunoprecipitation, immunofluorescence (frozen), and flow cytometry.

Western blot

WT, CAPN1-/- and CAPN2-/- mESC in day 8 embryonic body differentiation into NPCs.

The article lists a wrong catalog number 5239S instead of the correct catalog number 2539.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.