This is a knockout-validated antibody summary, based on the publication "Regulation of TET protein stability by calpains", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of WT, CAPN1-/- and CAPN2-/- cells. Tubulin was used as loading control. Tubulin was used as loading control. From [1].

Rabbit polyclonal IgG

Company: Thermo

Antibody: Calpain1

Catalog number: PA5-27681

Summary: Rabbit polyclonal IgG against a recombinant fragment corresponding to a region within amino acids 317 and 493 of human calpain 1. Reacts with human protein by western blot, immunocytochemistry, immunofluorescence, immunohistochemistry (paraffin), and immunoprecipitation.

Western blot

WT, CAPN1-/- and CAPN2-/- mESC.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.