This is a knockout-validated antibody summary, based on the publication "Enzymatic cleavage of myoferlin releases a dual C2-domain module linked to ERK signalling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1 (kappa light chain)
Company: Santa Cruz
Antibody: Calpain reg
Catalog number: sc-32325
Summary: Mouse monoclonal IgG1 (kappa light chain) against bovine myocardial small regulatory subunit complexed with calpain 2. Recommended for detection of Calpain reg of mouse, rat and human origin by western blot, immunoprecipitation and immunofluorescence. Non cross-reactive with the large calpain catalytic subunits.
Western blot
WT and CAPNS1−/− HEK293 and MEF cells.
5% skim milk in PBS + 1% Tween.
1:500 dilution.
Western blot analysis of CAPNS1 in WT and CAPNS1−/− HEK293 (b) and MEF (c) cells. Please see Figure 6b,c in the article [1].
The same clone (P1) is sold as Santa Cruz Biotechnology sc-32325; EMD Millipore MAB3083.
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