CaMKKβ antibody | knockout validation | Santa Cruz sc-100364

This is a knockout-validated antibody summary, based on the publication "CAMKK2 regulates mitochondrial function by controlling succinate dehydrogenase expression, post-translational modification, megacomplex assembly, and activity in a cell-type-specific manner", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CaMKKβ antibody | knockout validation | Santa Cruz sc-100364 figure 1
Figure 1. Immunoblots showing expression of CAMKK2 in HEK293 and HepG2 cells. WT: wild-type (parental), KO: CAMKK2 knockout (CAMKK2−/−), M: molecular weight ladder, and ns: nonspecific band. From [1].
Antibody information

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: CaMKKβ

Catalog number: sc-100364

Summary: Mouse monoclonal antibody against recombinant human CaMKKβ. Recommended for detection of CaMKKβ of human and rat origin by western blot, immunoprecipitation and ELISA.

Validation Method

Western blot

Sample

WT and CAMKK2 KO HEK293 and HepG2 cells.

Blocking agent

EveryBlot Blocking Buffer (Bio-Rad, Cat No: 12010947).

Primary incubation

1:1,000 dilution.

Detection

Chemiluminescence, imaged with the ChemiDoc MP Imaging System (Bio-Rad).

References
  1. Sabbir M, Taylor C, Zahradka P. CAMKK2 regulates mitochondrial function by controlling succinate dehydrogenase expression, post-translational modification, megacomplex assembly, and activity in a cell-type-specific manner. Cell Commun Signal. 2021;19:98 pubmed publisher