CYP2E1 antibody | knockout validation | Abcam ab84598
DOI
//dx.doi.org/10.13070/ko.en.7.2174
Date
2017-03-16

This is a knockout-validated antibody summary, based on the publication "Characterization of novel cytochrome P450 2E1 knockout rat model generated by CRISPR/Cas9", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit polyclonal IgG

Company: Abcam

Antibody: CYP2E1

Catalog number: ab84598

Summary: Rabbit polyclonal IgG against a synthetic peptide derived from within residues 400 to the C-terminus of human Cytochrome P450 2E1. Reacts with human and rat. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin). Predicted to work with cat, macaque monkey, and orangutan.

Validation Method

Western blot

Sample

Microsomes prepared from WT and Cyp2e1-KO rat livers.

Blocking agent

5% skim milk powder in PBS buffer (containing 0.1% tween-20) for 1 hour.

Primary incubation

1:1,000 dilution in commercial antibody diluent, overnight at 4°C.

Secondary incubation

1:10,000 dilution anti-rabbit secondary antibody in commercial antibody diluent.

Detection

Odyssey imager system (LICOR, USA).

Figure

Please see Figure 4b in the article [1].

References
  1. Wang X, Tang Y, Lu J, Shao Y, Qin X, Li Y, et al. Characterization of novel cytochrome P450 2E1 knockout rat model generated by CRISPR/Cas9. Biochem Pharmacol. 2016;105:80-90 pubmed publisher