This is a knockout-validated antibody summary, based on the publication "Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of CTU2 knockout in NOZCas9 cells. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: CTU2/NCS2

Catalog number: ab177160

Summary: Rabbit monoclonal IgG against a synthetic peptide within human CTU2/NCS2 (aa 300-400, Cysteine residue). Reacts with mouse, rat and human. Suitable for western blot, immunohistochemistry (paraffin) and flow cytometry. Unsuitable for immunocytochemistry or immunoprecipitation.

Western blot

Control and CTU2-KO NOZ cells. Cell lysates were prepared in radioimmunoprecipitation lysis buffer (50 mm Tris, pH 7.4, 150 mm NaCl, 1% NP-40, 0.1% sodium dodecyl sulphate (SDS), 2 µm EDTA) containing proteinase inhibitor.

5% skim milk at room temperature for 1 h.

1:1,000 dilution at 4 °C overnight.

Horseradish peroxidase conjugated.

Signals were detected using the ECL Kit (Millipore).