This is a knockout-validated antibody summary, based on the publication "Hyperactivation of Mammalian Target of Rapamycin Complex 1 (mTORC1) Promotes Breast Cancer Progression through Enhancing Glucose Starvation-induced Autophagy and Akt Signaling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Tsc1, tuberous sclerosis 1.

The catalog number is 4906S

Rabbit polyclonal antibody detects endogenous levels of total hamartin/TSC1 protein from human, mouse and rat origin. Has been successfully used in western blot and immunoprecipitation.

Figure 1. Immunoblotting of Ctrl and Tsc1 KO mouse primary mammary tumor cells. From Figure 1 [1].

Cell Signaling Technology

Western blot

Primary mouse mammary tumor cells Tsc1 KO PyMT, CreER. Tumor cells or frozen tumor tissues that had been ground with a ceramic mortar and pestle on dry ice using Nonidet P-40 lysis buffer (5 ml of Nonidet P-40, 10 ml of 1 m Tris-HCl (pH 8), 4 g of NaCl, 50 ml of glycerol, 500 ml of 1 m Na3VO4, and 435 ml of double-distilled water) in the presence of protease inhibitor mixture (Sigma, catalog no. P2714) by sonication.

Deletion of Tsc1 and consequent activation of mTORC1 promoted mammary tumor growth and metastasis.