CST complex subunit CTC1 antibody | knockout validation | Millipore MABE1103

This is a knockout-validated antibody summary, based on the publication "CTC1-mediated C-strand fill-in is an essential step in telomere length maintenance", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CST complex subunit CTC1 antibody | knockout validation | Millipore MABE1103 figure 1
Figure 1. Western blot showing loss of CTC1 from CTC1−/− cells. Blot was probed with antibody to CTC1 or actin as a loading control. Arrow: CTC1, *: cross-reacting bands. From [1].
Antibody information

Mouse monoclonal IgG2b

Company: Millipore

Antibody: CST complex subunit CTC1

Catalog number: MABE1103

Summary: Mouse monoclonal IgG2b against a linear peptide corresponding to an N-terminal region sequence of human CTC1. Reacts with human by western blot.

Validation Method

Western blot

Sample

Cells were lysed in NP-40 lysis buffer (20 mM Tris pH8.0, 100 mM NaCl, 1 mM MgCl2 and 0.1% NP-40).

Blocking agent

5% milk.

Secondary incubation

HRP-conjugated secondary antibody.

Detection

Western Lightning ECL (Perkin Elmer) or Supersignal ECL (Thermo).

Notes

Antibody is not very specific, but image of Millipore web shows same unspecific bands..

References
  1. Feng X, Hsu S, Kasbek C, Chaiken M, Price C. CTC1-mediated C-strand fill-in is an essential step in telomere length maintenance.. Nucleic Acids Res. 2017;45:4281-4293 pubmed publisher