CRMP2 antibody | knockout validation | Epitomics 5799-1
DOI
//dx.doi.org/10.13070/ko.en.7.1958
Date
2017-01-14

This is a knockout-validated antibody summary, based on the publication "Brain-specific Crmp2 deletion leads to neuronal development deficits and behavioural impairments in mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CRMP2 antibody | knockout validation | Epitomics 5799-1 figure 1
Figure 1. From Figure 1 in [1]. Western blot of lysates from various brain regions of wild-type (Ctrl) and CRMP2 conditional knockout (cKO) mice. GAPDH serves as a loading control.
Antibody information

Recombinant rabbit monoclonal

Company: Epitomics (abcam)

Antibody: CRMP2

Catalog number: 5799-1 (Clone EPR7876), now Abcam (ab129066).

Summary: Recombinant rabbit monoclonal antibody.

Specifically recognises CRMP2 phosphorylated on T514.

Supplier recommended for WB. Stated to be unsuitable for IP or IHC.

Reacts with human, mouse and rat phospho-T514 CRMP2.

Validation Method

Western blot

Sample

Lysates of brain regions from wild-type and CRMP2 conditional knock out mice.

Primary incubation

Anti-CRMP2 (phospho-T514) diluted 1:1000.

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Zhang H, Kang E, Wang Y, Yang C, Yu H, Wang Q, et al. Brain-specific Crmp2 deletion leads to neuronal development deficits and behavioural impairments in mice. Nat Commun. 2016;7: pubmed publisher