CRMP2 antibody | knockout validation | Abcam ab129082

This is a knockout-validated antibody summary, based on the publication "Neuronal Conditional Knockout of Collapsin Response Mediator Protein 2 Ameliorates Disease Severity in a Mouse Model of Multiple Sclerosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CRMP2 antibody | knockout validation | Abcam ab129082 figure 1
Figure 1. Immunohistochemical staining of motor CTX for CRMP2 (green) and CTIP2 (red) to label descending motor neurons. Please note the KO is partial. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: CRMP2

Catalog number: ab129082

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues near the C terminus of human CRMP2. Reacts with mouse, rat and human. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunocytochemistry/immunofluorescence and flow cytometry.

Validation Method

Immunohistochemistry

Sample

WT and CRMP2 cKO mouse brain cortex.

Primary incubation

1:1,000 dilution overnight at 4°C.

Secondary incubation

1:1,000 dilution fluorescein (FITC)- conjugated secondary antibodies (Vector Laboratories) in blocking solution.

Detection

Zeiss Axioplan 2 microscope equipped with an MRm camera using a 40× objective.

Clone note

The same clone (EPR7792) is sold as LifeSpan Biosciences LS-B9303; Abcam ab129082.

References
  1. Moutal A, Kalinin S, Kowal K, Marangoni N, Dupree J, Lin S, et al. Neuronal Conditional Knockout of Collapsin Response Mediator Protein 2 Ameliorates Disease Severity in a Mouse Model of Multiple Sclerosis. ASN Neuro. 2019;11:1759091419892090 pubmed publisher