This is a knockout-validated antibody summary, based on the publication "The role of fatty acid β-oxidation in lymphangiogenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Micrographs of the jugular lymph sacs (JLS) of embryonal day (E) 14.5 wild type mice (left) and mice with lymphendothelial-specific Cpt1a gene knockout (right), stained for CPT1a (magenta). Dotted lines denote endothelium in the JLS. Arrows in the right panel denote lymphendothelial cells of the JLS without CPT1a immunoreactivity. Scale bars represent 100 µm. Courtesy of the Author.

Mouse monoclonal IgG2b kappa

Company: Abcam

Antibody: CPT1A

Catalog number: ab128568

Summary: Mouse monoclonal IgG2b kappa against a recombinant fragment corresponding to amino acids 489-773 of human CPT1A. Reacts with mouse, rat and human. Suitable for western blot, immunohistochemistry (paraffin), immunocytochemistry/immunofluorescence, flow cytometry and ELISA.

Figure 2. Micrographs of the jugular lymph sacs (JLS) of embryonal day (E) 14.5 wild type mice (left) and mice with lymphendothelial-specific Cpt1a gene knockout (right), stained for CPT1a (magenta) and VEGFR3 (green; visualizing the lymphendothelial cells). For the CPT1a signal channel only (large panels), a higher magnification is shown than for the VEGFR3 signal channel (top small panels) or the merged signal (bottom small panels). Dotted lines denote endothelium in the JLS. Arrows denote lymphendothelial cells of the JLS without CPT1A immunoreactivity. VEGR3 antibody: anti-VEGFR3/Flt4 (R&D, AF743). Scale bars represent 100 µm. Courtesy of the Author.

Immunohistochemistry

Wild-type (Prox1) and Prox1∆CPT1A mouse embryos at E14. Embryos were fixed overnight in 1% paraformaldehyde at 4°C, then washed with PBS before embedding in paraffin for sectioning.

Appropriate AlexaFluor-405, -488, -568 or -647 conjugated secondary antibodies were used (Molecular Probes). Tyramide signal amplification (TSA) kit was used as per manufacturer’s specifications (Perkin Elmer).

Confocal microscope images were captured of the dorsal region of cross sections of mouse embryos using a Zeiss LSM 780 confocal microscope (objectives: 310 with numerical aperture (NA) 0.3, 320 with NA 0.4; Carl Zeiss).