This is a knockout-validated antibody summary, based on the publication "Elimination of the male reproductive tract in the female embryo is promoted by COUP-TFII in mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2A
Company: R&D Systems
Antibody: COUP-TF II/NR2F2
Catalog number: PP-H7147-00
Summary: Mouse monoclonal IgG2A against recombinant human COUP-TF II/NR2F2 (amino acids 43-64). Recognizes human and rat protein. Suitable for western blot, immunoprecipitation and immunohistochemistry.
Immunohistochemistry
Control and Coup-tfII knockout mouse embryos.
5% donkey serum in PBST for 1 hour.
1:300 in blocking solution overnight at 4°C.
1:200 dilution Alexa Fluor@ 488 anti-mouse IgG (Invitrogen).
Leica DMI4000 confocal microscope.
Double immunofluorescence of COUP-TFII (green) and β-GAL (red) in the mesonephroi of control (C-E) and Coup-tfII knockout (F-H) XX embryos at E12.5, 14.5, and 16.5. N=3 per group in A and C-H. The blue, magenta and white dotted circles indicate Wolffian ducts (remnants at E16.5), Müllerian ducts, and mesonephric tubules, respectively. Scale bar: 50 μm. Please see Suppl. Figure 1 in the article [1].
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