COMMD1 antibody | knockout validation | R&D Systems MAB7526
DOI
//dx.doi.org/10.13070/ko.en.6.1923
Date
2016-12-12

This is a knockout-validated antibody summary, based on the publication "COMMD1 is linked to the WASH complex and regulates endosomal trafficking of the copper transporter ATP7A", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

COMMD1 antibody | knockout validation | R&D Systems MAB7526 figure 1
Figure 1. From figure 2 in [1]. Western blot of lysates from COMMD1 deleted mouse fibroblasts and isogenic control cells were probed with anti-COMMD1 antibody. Actin serves as a loading control.
Antibody information

Mouse monoclonal

Company: R&D Systems

Antibody: COMMD1

Catalog number: MAB7526

Summary: Mouse monoclonal antibody raised against an E.coli-expressed recombinant protein containing residues 37-135 of human COMMD1.

Supplier recommended for WB, ICC and elisa. Reacts with human COMMD1.

Validation Method

Western blot

Sample

Lysates of COMMD1 deleted mouse fibroblasts and isogenic control cells.

Primary incubation

Anti-COMMD1 antibody.

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Phillips-Krawczak C, Singla A, Starokadomskyy P, Deng Z, Osborne D, Li H, et al. COMMD1 is linked to the WASH complex and regulates endosomal trafficking of the copper transporter ATP7A. Mol Biol Cell. 2015;26:91-103 pubmed publisher