COMMD1 antibody | knockout validation | Proteintech 11938-1-AP

DOI

//dx.doi.org/10.13070/ko.en.6.1642

Date

2016-09-24

This is a knockout-validated antibody summary, based on the publication "CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

COMMD1 antibody | knockout validation | Proteintech 11938-1-AP figure 1

Figure 1. Dog: Western blot analysis of COMMD1 in livers from an unaffected dog (COMMD1+/+) and an affected dog homozygous for a loss-of-function mutation in COMMD1 (COMMD1-/-). Actin is used as loading control, from [1].

Antibody information

Antibody: COMMD1

Catalog number: 11938-1-AP

Summary: Rabbit polyclonal raised against recombinant human protein. Detects human, mouse, rat and dog in western blot, immunofluorescence, immunhistochemistry, immunoprecipitation and ELISA.

COMMD1 antibody | knockout validation | Proteintech 11938-1-AP figure 2

Figure 2. Mouse: COMMD1 levels in Commd1f/f and in Commd1-/- MEFs determined by immunoblot analysis. Tubulin was used as loading control. From [1].

Company: Proteintech

Validation Method

WB, Western blot

Sample

livers from an unaffected dog (COMMD1+/+) and an affected dog homozygous for a loss-of-function mutation in COMMD1 (COMMD1-/-_ and COMMD1 levels in Commd1f/f and in Commd1-/-MEFs.

Primary incubation

1:1,000

References

Bartuzi P, Billadeau D, Favier R, Rong S, Dekker D, Fedoseienko A, et al. CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL. Nat Commun. 2016;7:10961 pubmed publisher

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