This is a knockout-validated antibody summary, based on the publication "The Cyclic Phosphodiesterase CNP and RNA Cyclase RtcA Fine-Tune Noncanonical XBP1 Splicing During ER Stress", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2b (kappa light chain)
Company: Santa Cruz
Antibody: CNPase
Catalog number: sc-166019
Summary: Mouse monoclonal IgG2b (kappa light chain) against amino acids 121-420 mapping at the C-terminus of mouse CNPase. Recommended for detection of CNPase isoforms 1 and 2 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.
Western blot
WT and CNP−/− mouse ES cells.
Horseradish peroxidase–conjugated secondary antibodies (Santa Cruz Biotechnology and Bio-Rad).
A CNP−/− ES cell clone was identified with frameshift mutations in both alleles. Western blotting showed the loss of CNP protein. Please see Figure 5b in the article [1].
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