This is a knockout-validated antibody summary, based on the publication "CNOT3-Dependent mRNA Deadenylation Safeguards the Pluripotent State", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Induction of Cnot3 deletion in Cnot3 cKO ESCs. Cells were treated with or without 4-OHT, and Cnot3 expression was determined by western blot at the indicated time points. From [1].

Mouse monoclonal IgG1 Kappa

Company: Abnova

Antibody: CNOT3

Catalog number: H00004849-M01

Summary: Mouse monoclonal IgG1 Kappa raised against a partial recombinant human CNOT3 (1 a.a. ~ 100 a.a). Reacts with human protein by western blot, immunofluorescence and ELISA.

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Figure 2. Immunofluorescence staining of CNOT3 in WT and Cnot3 deletion embryos at the indicated developmental stages. Scale bars, 20 um. From [1].

Western blot | Immunocytochemistry

WT and Cnot3-KO embryos and ESCs. IC: Embryos were fixed in 4% formaldehyde in PBS.

IC: goat anti-mouse-488 (A11001, Life Technologies).

IC: Image data were acquired with a Zeiss LSM 780 microscope.

Immunofluorescence of an embryo could be arguably IC rather than IHC, since no sectioning is required and the small amount of cells present.

The same clone (4B8) is sold as Abnova H00004849-M01, H00004849-M01A; Sigma-Aldrich WH0004849M1.