CLIM-1 antibody | knockout validation | Santa Cruz sc-101088

This is a knockout-validated antibody summary, based on the publication "LDB2 inhibits proliferation and migration in liver cancer cells by abrogating HEY1 expression", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CLIM-1 antibody | knockout validation | Santa Cruz sc-101088 figure 1
Figure 1. LDB2 was deleted in HCC samples using two individual sgRNAs through CRISPR/Cas9 technology. The deletion efficiency was validated by Western blot. From [1].
Antibody information

Mouse monoclonal mouse IgG1 κ (kappa light chain)

Company: Santa Cruz

Antibody: CLIM-1

Catalog number: sc-101088

Summary: Mouse monoclonal mouse IgG1 κ (kappa light chain) against recombinant human CLIM-1 (LDB2). Recommended for detection of CLIM-1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Validation Method

Western blot

Sample

WT and LDB2-KO human hepatocellular carcinoma (HCC) cells.

References
  1. Yu H, Jia R, Zhao L, Song S, Gu J, Zhang H. LDB2 inhibits proliferation and migration in liver cancer cells by abrogating HEY1 expression.. Oncotarget. 2017;8:94440-94449 pubmed publisher