CLIC1 antibody | knockout validation | Santa Cruz sc-81873

This is a knockout-validated antibody summary, based on the publication "CLIC1 Inhibition Attenuates Vascular Inflammation, Oxidative Stress, and Endothelial Injury", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CLIC1 antibody | knockout validation | Santa Cruz sc-81873 figure 1
Figure 1. Western blot of CLIC1 in control and CLIC1 knockout HUVECs cells. β-actin was used as a loading control. From [1].
Antibody information

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: CLIC1

Catalog number: sc-81873

Summary: Mouse monoclonal IgG2a (kappa light chain) against recombinant human CLIC1. Recommended for detection of CLIC1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Validation Method

Western blot

Sample

Control and CLIC1-KO HUVEC cells. Total protein was extracted by Membrane and Cytosol Protein Extraction Kit (P0033, Beyotime, China).

Blocking agent

5% skim milk for 2 h.

Primary incubation

At 4˚C overnight.

Secondary incubation

HRP-conjugated secondary antibody for 2 h.

Detection

ECL-plus detection system (Clinx Science Instruments, China).

References
  1. Xu Y, Zhu J, Hu X, Wang C, Lu D, Gong C, et al. CLIC1 Inhibition Attenuates Vascular Inflammation, Oxidative Stress, and Endothelial Injury. PLoS ONE. 2016;11:e0166790 pubmed publisher