This is a knockout-validated antibody summary, based on the publication "Cytokeratin 18 is not required for morphogenesis of developing prostates but contributes to adult prostate regeneration", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunofluorescence staining of CK18 in frozen sections of WT and CK18−/− prostates. Representative images are shown. From [1].

Rabbit monoclonal

Company: Epitomics, now part of Abcam

Antibody: CK18

Catalog number: 3258-1, the antibody is the same as Abcam ab133263.

Summary: Rabbit monoclonal against a synthetic peptide corresponding to a region within the C-terminal end of human cytokeratin 18. Reacts with rat, and human. Recommended for western blot, immunohistochemistry (frozen and paraffin), immunocytochemistry, but not suitable for flow cytometry or immunoprecipitation.

Immunohistochemistry

8 um cryosections of prostates from WT and CK18−/− mice

10% normal goat serum (NGS) for 1 hour at room temperature

overnight at 4°C

Secondary antibodies conjugated with Alexa-488 or 594 (Jackson Laboratory), 1 hour at room temperature

Nikon fluorescent microscope (Nikon Eclipse Ti) with 400x magnification

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.