This is a knockout-validated antibody summary, based on the publication " Motor phenotypes and molecular networks associated with germline deficiency of Ciz1", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit polyclonal

Company: Bethyl Laboratories

Antibody: CIZ1

Catalog number: A301-496A

Summary: Rabbit polyclonal antibody raised against a peptide corresponding to residues 325-375 of human CIZ1.

Supplier recommended for WB and IP.

Antibody is epitope affinity purified.

Reacts with human CIZ1.

Immunohistochemistry

Mouse brain sections fixed with 4% paraformaldehyde.

PBS containing 2% milk powder and 0.3% Triton X-100

Anti-CIZ1 diluted 1:500.

Biotinylated goat anti-rabbit IgG (Jackson ImmunoResearch Labs) for 4 hours.

VECTAstain ABC kit (Vecta Labs) with DAB.

See Supplemental Figures 6 & 7 in [1].

The authors commented that there was always a degree of non-specific immunoreactivity in samples from CIZ1 knockout animals.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.