This is a knockout-validated antibody summary, based on the publication " Motor phenotypes and molecular networks associated with germline deficiency of Ciz1", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse polyclonal
Company: Abcam
Antibody: CIZ1 (CDKN1A interacting zinc finger protein 1)
Catalog number: Ab172442
Summary: Mouse polyclonal antibody raised against full-length recombinant human CIZ1. Supplier recommended for WB. Protein A purified. Reacts with human CIZ1.
Western blot (with initial immunoprecipitation step)
Lysates of mouse brain cerebellum and cerebral cortex immunoprecipitated with anti-CIZ1 antibody.
5% milk powder.
Anti-CIZ1 diluted 1:10000.
HRP-conjugated goat anti-rabbit IgG (Jackson ImmunoResearch Labs).
ECL reagent (Amersham).
See figure 3 in [1].
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.