This is a knockout-validated antibody summary, based on the publication "Mutant α2-chimaerin signals via bidirectional ephrin pathways in Duane retraction syndrome", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: CHN 1
Catalog number: ab156869
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues in human CHN 1. Reacts with mouse, rat, and human. It is predicted to work with chicken. Suitable for western blot and immunoprecipitation. Unsuitable for immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) or flow cytometry.
Western blot
Brain from Chn1WT/WT, Chn1WT/KO and Chn1KO/KO mice. Postnatal day 0-2 whole brain was homogenized on ice in RIPA buffer (Thermo Scientific), 1mM EDTA (Gibco), and Halt protease and phosphatase inhibitor cocktail (Thermo Scientific).
5% milk in PBST.
1:1,000 dilution overnight at 4 C.
1:5,000 dilution peroxidase AffiniPure donkey anti-rabbit IgG (Jackson ImmunoResearch).
Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) and imaged with a FujiFilm LAS-4000 with CCD camera (GE Healthcare) and MultiGauge software (GE Healthcare).
Western blot of a2-chimaerin and GADPH from P0-2 brain of littermate Chn1WT/WT, Chn1WT/KO and Chn1KO/KO mice. Please see Suppl. Figure 1d in the article [1].
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