This is a knockout-validated antibody summary, based on the publication "CHD7 maintains neural stem cell quiescence and prevents premature stem cell depletion in the adult hippocampus", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot for CHD7 on total cell lysates of cultured fetal-derived NSCs from Chd7null (NestinCre;Chd7-/-) and control (Chd7+/+) cells. Cleavage and polyadenylation specificity factor 100 (CPSF100) was used as a loading control. From [1].

Rabbit polyclonal

Company: Abcam

Antibody: CHD7

Catalog number: ab31824

Summary: Rabbit polyclonal against a synthetic peptide (conjugated to KLH) derived from within residues 2950 to the C-terminus of mouse Chd7. Predicted to work with mouse and human, since it shows high titre values against the immunizing peptide by ELISA. Suitable for immunohistochemistry.

Western blot | Immunohistochemistry

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Figure 2. Representative image of a section of the subgranular zone stained with antibodies raised against CHD7 and ASCL1 in control (E) and Chd7null (F) adult brains 4 weeks after TAM administration. Sections were stained with antibodies against ASCL1 to identify the neural stem/progenitor cell population that normally express high levels of CHD7. From [1].

WB: cultured fetal-derived NSCs from Chd7null (NestinCre;Chd7-/-) and control (Chd7+/+) mouse cells lysed in 8 M urea, 1% CHAPS, 50 mM TRIS pH7.9.

IHC: Coronal mouse brain sections (20-µm-thick) from GLAST::CreERT2;Chd7f/f mouse.

WB: 0.5% nonfat milk powder in TBS with 0.5% Tween 20 (TBST).

IHC: 10% heat-inactivated goat serum (GS) for 1 hour.

WB: 1/2,000 in 5% BSA, TBST overnight at 4°C.

IHC: 1/80 in 5% GS in 0.1% Triton X (Sigma) in PBS overnight at 4°C.

WB: horseradish peroxidase (HRP)-conjugated secondary antibody (Millipore, U.K.) for 1 hour at room temperature.

IHC: Alexa-Fluor-labeled secondary antibodies diluted at 1/200 (Life Technologies) for 1 hour.

WB: Clarity Western ECL reagent (Bio-rad, U.K.) and a Bio-Rad gel doc system.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.