CHD1L antibody | knockout validation | Cell Signaling 13460

This is a knockout-validated antibody summary, based on the publication "Defective ALC1 nucleosome remodeling confers PARPi sensitization and synthetic lethality with HRD", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CHD1L antibody | knockout validation | Cell Signaling 13460 figure 1
Figure 1. CRISPR-mediated inactivation of ALC1 in eHAP. Immunoblot of WCEs in ALC1+/+ and ALC1−/− cells, probed for ALC1. hnRNPA1 was used as a loading control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: CHD1L

Catalog number: 13460

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Gly231 of human CHD1L protein (ALC1). Reacts with human by western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and ALC1−/− eHAP cells.

Blocking agent

5% skim milk/TBST (TBS/0.1%Tween-20) for 1 h at room temperature.

Primary incubation

Overnight at 4°C.

Secondary incubation

Horseradish peroxidase conjugated (HRP) for 1 h at room temperature.

Detection

Clarity or Clarity Max Western ECL Substrate (Bio-Rad).

References
  1. Hewitt G, Borel V, Segura Bayona S, Takaki T, Ruis P, Bellelli R, et al. Defective ALC1 nucleosome remodeling confers PARPi sensitization and synthetic lethality with HRD. Mol Cell. 2020;: pubmed publisher