This is a knockout-validated antibody summary, based on the publication "Osteoblast CFTR inactivation reduces differentiation and osteoprotegerin expression in a mouse model of cystic fibrosis-related bone disease", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Calvariae were harvested from Cftr+/+ and Cftr−/− 4-day-old pups, fixed, sectioned immunostained for CFTR using the 3G11 rat anti-CFTR monoclonal antibody and counterstained with hematoxylin. From [1].

Rabbit monoclonal

Company: Cystic Fibrosis Folding Consortium

Antibody: CFTR

Catalog number: 3G11

Immunohistochemistry

Calvariae from Cftr+/+ and Cftr−/− mice.

10 mg/ml BSA in PBS.

1∶500 dilution overnight at 4°C.

Anti-rat biotin-conjugated secondary antibody for 30 minutes.

Vectastain ABC Reagent (Vector Laboratories).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.