CELF-6 antibody | knockout validation | Abcam ab173282

This is a knockout-validated antibody summary, based on the publication "RNA-binding protein CELF6 is cell cycle regulated and controls cancer cell proliferation by stabilizing p21", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CELF-6 antibody | knockout validation | Abcam ab173282 figure 1
Figure 1. The protein levels of the indicated proteins were analyzed by immunoblotting. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: CELF-6

Catalog number: ab173282

Summary: Rabbit monoclonal IgG against a synthetic peptide within human CELF-6 (aa 300-400, Cysteine residue). Reacts with mouse, rat, and human. Suitable for western blot, immunohistochemistry (paraffin), and flow cytometry. Unsuitable for immunocytochemistry/immunofluorescence or immunoprecipitation.

Validation Method

Western blot

Sample

Control and CELF6 knockout in both HCT116 cells and HepG2 cells.

Blocking agent

5% nonfat milk.

Secondary incubation

HRP-conjugated secondary antibodies.

Detection

Chemiluminescence (Thermo Fisher Scientific) and exposed to X-ray films.

References
  1. Liu G, Zhang Q, Xia L, Shi M, Cai J, Zhang H, et al. RNA-binding protein CELF6 is cell cycle regulated and controls cancer cell proliferation by stabilizing p21. Cell Death Dis. 2019;10:688 pubmed publisher