CEBP Alpha/CEBPA antibody | knockout validation | Abcam ab40761

This is a knockout-validated antibody summary, based on the publication "CCAAT enhancer binding protein α suppresses proliferation, metastasis, and epithelial-mesenchymal transition of ovarian cancer cells via suppressing the Wnt/β-catenin signaling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CEBP Alpha/CEBPA antibody | knockout validation | Abcam ab40761 figure 1
Figure 1. A2780 and OV-90 cells were transduced with sg-CEBPA-1, sg-CEBPA-2, or sg-NC, then relative CEBPA expression was evaluated by western blot. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: CEBP Alpha/CEBPA

Catalog number: ab40761

Summary: Rabbit monoclonal antibody against a synthetic peptide within Human CEBP Alpha/CEBPA aa 1-100 (N terminal). Reacts with human. Suitable for western blot, immunocytochemistry/immunofluorescence, and flow cytometry (Intra). Unsuitable for immunoprecipitation or immunohistochemistry.

Validation Method

Western blot

Sample

Control and CEBPA-KO A2780 and OV-90 cells.

Primary incubation

1:1,000 dilution at 4 °C overnight

Secondary incubation

1:4,000 dilution goat anti-rabbit IgG HRP-linked antibody (Cell Signaling, 7074) for 1 h at room temperature.

References
  1. Zhang L, Li M, Tian C, Wang T, Mi S. CCAAT enhancer binding protein α suppresses proliferation, metastasis, and epithelial-mesenchymal transition of ovarian cancer cells via suppressing the Wnt/β-catenin signaling. Neoplasma. 2021;68:602-612 pubmed publisher