CDK6 antibody | knockout validation | ThermoFisher AHZ0232

This is a knockout-validated antibody summary, based on the publication "A natural WNT signaling variant potently synergizes with Cdkn2ab loss in skin carcinogenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CDK6 antibody | knockout validation | ThermoFisher AHZ0232 figure 1
Figure 1. Western blot analysis of CDK6 expression validating Cdk6 CRISPR/Cas inactivation and transduced CDK6 overexpression. From [1].
Antibody information

Mouse monoclonal IgG1

Company: ThermoFisher

Antibody: AHZ0232

Catalog number: CDK6

Summary: Mouse monoclonal IgG1 against purified recombinant cdk6 protein. Reacts with human, mouse, adn rat. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin and frozen), and immunoprecipitation.

Validation Method

Western blot

Sample

Control and Cdk6-KO skin tumour cell lines (derived from primary cultures).

Primary incubation

1:1,000 dilution.

Secondary incubation

Goat anti-mouse (AMI3404, Biosource) antibodies coupled to horseradish peroxidase.

Detection

Enhanced chemiluminescence (Pierce 34080).

References
  1. Krimpenfort P, Snoek M, Lambooij J, Song J, van der Weide R, Bhaskaran R, et al. A natural WNT signaling variant potently synergizes with Cdkn2ab loss in skin carcinogenesis. Nat Commun. 2019;10:1425 pubmed publisher