This is a knockout-validated antibody summary, based on the publication "Cyclin D-CDK4 kinase destabilizes PD-L1 via cullin 3-SPOP to control cancer immune surveillance", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1
Company: Cell Signaling
Antibody: CDK6
Catalog number: 3136
Summary: Mouse monoclonal IgG1 against a recombinant human CDK6 corresponding to amino acids 30-65. Reacts with human, mouse and rat by western blot.
Western blot
Wild type and cdk6−/− MEFs. Cells were lysed in EBC buffer (50 mM Tris pH 7.5, 120 mM NaCl, 0.5% NP-40) supplemented with protease inhibitors (Complete Mini, Roche) and phosphatase inhibitors (phosphatase inhibitor cocktail set I and II, Calbiochem).
Peroxidase-conjugated anti-mouse secondary antibody (Sigma, A-4416).
Immunoblot analysis of whole cell lysates derived from wild type and cdk6−/− MEFs. Please see Extended Data Figure 2h in the article [1].
The same clone (DCS83) is sold as Santa Cruz Biotechnology sc-53638; LifeSpan Biosciences LS-C179591, LS-C193423; Cell Signaling Technology 3136.
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