CDK1 antibody | knockout validation | Abcam ab18

This is a knockout-validated antibody summary, based on the publication "Loss of hepatocyte cell division leads to liver inflammation and fibrosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CDK1 antibody | knockout validation | Abcam ab18 figure 1
Figure 1. Immunoblot of lysates from WT and Cdk1Liv-/- hepatoblasts (E14.5) and hepatocytes (P14) probed for CDK1 with HSP90 as loading control. From [1].
Antibody information

Mouse monoclonal IgG2a

Company: Abcam

Antibody: CDK1

Catalog number: ab18

Summary: Mouse monoclonal antibody against a recombinant fragment of mouse CDK1 (the epitope is thought to be residues 220-227 of mouse cdc2, LGTPNNEV). Reacts with mouse and human. It is predicted to work with rat, chicken, and Xenopus laevis. Suitable for western blot, immunohistochemistry (paraffin) and flow cytometry.

Validation Method

Western blot

Sample

WT and Cdk1Liv-/- mouse hepatocytes.

Primary incubation

1:1,000 dilution.

Clone note

The same clone (A17) is sold as Abcam ab18, ab187804, ab203852; ImmuQuest IQ596FITC; Invitrogen 33-1800; Boster MA1077.

References
  1. Dewhurst M, Ow J, Zafer G, Van Hul N, Wollmann H, Bisteau X, et al. Loss of hepatocyte cell division leads to liver inflammation and fibrosis. PLoS Genet. 2020;16:e1009084 pubmed publisher