This is a knockout-validated antibody summary, based on the publication "Discovery of potent and selective MRCK inhibitors with therapeutic effect on skin cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1,kappa
Company: Abnova
Antibody: CDC42BPA
Catalog number: H00008476-M01
Summary: Mouse monoclonal IgG1,kappa against a partial recombinant CDC42BPA (551 a.a. ~ 650 a.a). Reacts with human by western blot and ELISA.
Western blot
Control and MRCKαβ double knockout MDA MB 231 cells.
Goat anti-mouse IgG Dylight 800 (35521; Thermo Scientific) or goat anti-mouse IgG IR Dye 680 (926-68020; LI-COR Biosciences).
Western blots from parental and MRCKαβ double CRISPR/Cas9 knockout (KO) MDA MB 231 cells that were blotted for total MRCK, αTubulin, MRCKα, MRCKβ, ERK2 or Cas9 as indicated. Please see Figure S4a in the article [1].
The same clone (1G7) is sold as LifeSpan Biosciences LS-C198572; Abnova H00008476-M01; Sigma-Aldrich WH0008476M1.
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