This is a knockout-validated antibody summary, based on the publication "RhoA, Rac1, and Cdc42 differentially regulate αSMA and collagen I expression in mesenchymal stem cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1
Company: BD Biosciences
Antibody: CDC42
Catalog number: 610929
Summary: Mouse monoclonal IgG1 against human CDC42 (aa. 1-191). Reacts with human, mouse, rat, and dog. Suitable for western blot. Not recommended for immunofluorescence
Western blot
WT and Cdc42-KO mesenchymal stem cells.
5% milk in TBS, 0.05% Tween (TBS-T) for 60 min.
Overnight at 4 °C.
Corresponding secondary antibodies (Jackson Immunoresearch) for 60 min.
Luminata Western horseradish peroxidase chemiluminescence substrate detection reagent (Millipore) for 1 min at room temperature. Luminescence was detected with medical X-ray film (Agfa).
Representative Western blotting of the indicated proteins in mesenchymal stem cells with knockout of Cdc42. Please see Figure 3a in the article [1].
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.