This is a knockout-validated antibody summary, based on the publication "Genetic Disruption of the Multifunctional CD98/LAT1 Complex Demonstrates the Key Role of Essential Amino Acid Transport in the Control of mTORC1 and Tumor Growth", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of lysates from WT and CD98 -/- LS174T cells. Probed with anti-CD98 (upper panel). Tubulin serves as a loading control. From [1].

Rabbit polyclonal

Company: Santa Cruz Biotechnology

Antibody: CD98

Catalog number: SC-9160

Summary: Rabbit polyclonal antibody raised against residues 230-529 of human CD98. Supplier recommended for WB, IP, IF, IHC and elisa. Reacts with rat, mouse and human CD98.

Note: this antibody will be discontinued December 31, 2016.

Western blot

Lysates of wild-type and CD98 -/- LS174T human colon adenocarcinoma cells.

5% milk powder in TBS.

1:1000 dilution.

HRP-conjugated anti-rabbit IgG (Promega).

ECL (Merck Millipore) in conjunction with LI-COR Odyssey Imaging System.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.